The polymers used for the preparation of nanoparticles are natural polymers and synthetic polymers. The kinetics and extent of scavenging are fast and critical below certain sizes and depend on the degree of their surface hydrophilicit 8. Amphiphilic polyanhydride nanoparticles stabilize bacillus anthracis protective antigen. Ultrasound is used to break the capsules which in turn release the dna thus eliciting an. Polyanhydrides general, suppliers, product, process. These will be resistant to digestion and degradation. Nanoparticles are currently made out of a very wide variety of materials, the most common of the new generation of nanoparticles being ceramics, which are best split into metal oxide ceramics, such as titanium, zinc, aluminum and iron oxides, to name a prominent few, and silicate nanoparticles silicates, or silicon oxides, are also. Polyanhydride nanoparticle interactions with host serum. Dec 07, 2011 nano particles, asbestos and synthetic mineral fibers are all emerging as highly toxic products of combustion with fatal consequences. This chapter concentrates on the polyanhydride class of polymers. Combinatorial evaluation of in vivo distribution of polyanhydride particlebased platforms for vaccine delivery latrisha k.
The methods can include administering to an animal in need of such treatment an effective antimicrobial amount of a composition comprising polyanhydride microparticles or nanoparticles that encapsulate a. Cph nanoparticles are able to activate bovine apc and demonstrate that this response is largely. Polyanhydrides are a class of biodegradable polymers characterized by anhydride bonds that connect repeat units of the polymer backbone chain. Nanoparticles are molded in nanoscale molds fabricated from nonwetting, low surface energy polymeric materials. Ultrasound is used to break the capsules which in turn release the dna thus eliciting an immune response to fish due to the vaccination. This family of pattern recognition receptors expressed on innate immune cells, such as macrophages and dendritic cells, can be used to modulate immune responses.
Polyanhydride nanoparticles based on 1,6bispcarboxyphenoxyhexane and 1,8bispcarboxyphenoxy3,6dioxaoctane were used. Polyaniline as dispersion agent, nano silver polyaniline composite particles was prepared by insitu synthesis in dmf solution. Polyanhydrides are known to undergo surface erosion, a desired property to attain near zeroorder drug release profile. Polyanhydride micelles with diverse morphologies for shaperegulated cellular internalization and blood circulation.
Suspensions of particles were prepared in nanopure water in order to characterize the synthesized particles. In this work, the in vivo safety profile of carbohydratefunctionalized polyanhydride nanoparticles was analyzed following parenteral and. In this work, the in vivo safety profile of carbohydratefunctionalized polyanhydride nanoparticles was analyzed following. Particles can also be homogenized in scf cryomilled to further reduce particle size. Recently, due to their biocompatibility and biodegradability, the food and drug administration has approved the use of the polyanhydride derived from sebacic acid and 1,3bispcarboxyphenoxy propane as the carrier of. The invention provides compositions and methods to treat microbial infections in animals, to inhibit the replication of microbes in infected cells, and to kill pathogens in infected cells.
Nanoparticle filled polymeric systems for gas barrier and flame retardant properties by yingji wu may 20 polymer composite gas barrier and fire retardant properties were studied in this investigation. The nanoparticles can include pharmaceutical compositions, taggants, contrast agents, biologic drugs, drug compositions, organic materials, and the like. The effects of varying the nanoparticle composition and feed ratio on the encapsulation efficiency were evaluated. Owing to their safe degradation products, polyanhydrides. Efficacy of mucosal polyanhydride nanovaccine against. Polyanhydride nanoparticles were encapsulated into anionic phresponsive microparticle gels, allowing for nanoparticle encapsulation in acidic conditions and subsequent release in neutral ph conditions. Polyanhydride micelles with diverse morphologies for shape. These polyanhydride particles can be used for applications such as drug delivery andor vaccine delivery. Automated highthroughput synthesis of proteinloaded. Free microsoft excel viewer software can also be used to view the. Characterizing the antitumor response in mice treated with.
Nanoparticle fabrication methods, systems, and materials. Read polymer chemistry influences monocytic uptake of polyanhydride nanospheres, pharmaceutical research on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Nano particles are known to cross intestinal permeability. Mar 12, 2018 phdependent dissociation of prompt formulations to release polyanhydride nano particles was observed after suspending the microparticles in aqueous solution at ph 1. Manuscript received february 10th, 2000, revised may 11th, 2000. Mitochondriatargeted antioxidants were recently shown to alleviate oxidative damage. Pdf polymeric micro and nanoparticles as promising drug delivery. Modification of brittle polylactide by novel hyperbranched. Carbohydrate functionalization of nanoparticles allows for targeting of ctype lectin receptors.
Silver nanoparticles could also affect whole societies and ecosystems silver nanoparticles can inhibit many bacteria, including good bacteria silver nanoparticles can prevent photosynthesis in algae societal and ethical implications of nano silver. We hypothesize that the mechanism behind this dramatically enhanced killing of the macrofilaria is the ability of the polyanhydride nano particles to behave as a trojan horse and penetrate the. Abstractmetal and metal oxide particles are intensively pursued because of their prominence in different fields of applications in science and technology. Polyanhydride nanoparticle synthesis the polyanhydride particles were made using the emulsificationsolvent evaporation procedures developed and widely utilized to make plga nanoparticles. Combinatorial evaluation of in vivo distribution of. Hemagglutininbased polyanhydride nanovaccines against h5n1 influenza elicit protective virus neutralizing titers and cellmediated immunity kathleen a ross,1 hyelee loyd,2 wuwei wu,2 lucas huntimer,3 shaheen ahmed,4 anthony sambol,5 scott broderick,6 zachary flickinger,2 krishna rajan,6 tatiana bronich,4 surya mallapragada,1 michael j wannemuehler,3 susan carpenter,2 balaji. Amphiphilic polyanhydride nanoparticles stabilize bacillus anthracis protective antigen abstract advancements toward an improved vaccine against bacillus anthracis, the causative agent of anthrax, have focused on formulations composed of the protective antigen pa adsorbed to aluminum hydroxide. Pdf neuronal protection against oxidative insult by. Synthesis of copper oxide nanoparticles using simple. Hemagglutininbased polyanhydride nanovaccines against h5n1 influenza elicit protective virus neutralizing titers and cellmediated immunity kathleen a ross,1 hyelee loyd,2 wuwei wu,2 lucas huntimer,3 shaheen ahmed,4 anthony sambol,5 scott broderick,6 zachary flickinger,2 krishna rajan,6 tatiana bronich,4 surya mallapragada,1 michael j wannemuehler,3 susan carpenter,2 balaji narasimhan1. The polyanhydride particles that include cph have hydrophobic properties, they resists hydrolytic degradation in vivo, and they resist degradation at acidic ph. Polyanhydrides and polyesters biodegradable polymers.
Key examples of materials for immune engineering approach biomaterial notes refs intrinsic immunogenicity polymer particles polyd,llacticcoglycolic acid t cell stimulation is shape dependent 16,18gold nps particle shape and hydrophobicity alter cytokine secretion. Microsoft excel software is recommended for viewing the. Chemical and biological engineering theses and dissertations. Ravi kumar 1 department of chemistry, university of roorkee, india. It is synthesized by the dehydration of diacid molecules by melt polycondensation. The microstructure and characterization of the sample were studied by uvvis absorption spectroscopy infrared spectroscopy.
Nano fabricated drug delivery devices som shankar dubey, battula sreenivas rao department of chemistry, gitam institute of technology, gitam university, visakhapatnam 530045. In this work, we demonstrate that biodegradable amphiphilic polyanhydride nanoparticles, which have previously been shown to provide controlled antigen delivery, antigen stability, immune modulation, and protection in a single dose against a pathogenic challenge, can stabilize and release functional pa. Furthermore, in most cases of cancer therapy using nanocarriers, the payloads of therapeutic agents need to be released. Polyanhydride nanoparticle delivery platform dramatically enhances killing of filarial worms article pdf available in plos neglected tropical diseases 910. Nov 25, 2014 carbohydrate functionalization of nanoparticles allows for targeting of ctype lectin receptors. There was formation of new hyperbranched polymerbased crosslinked particles in the pla matrix.
Size distribution and surface charge were measured using a dynamic light scattering dls zetasizer nano zs instrument malvern, southborough, ma. In some embodiments, the process of fabricating the micro andor nano particles includes initially forming a replicate mold. The development of highthroughput techniques and combinatorial libraries can facilitate rapid synthesis and screening of biomaterialbased nanocarriers for drug and vaccine delivery. Their synthesis and ty pes saba hasan amity institute of biotechnology, amity university uttar pradesh lucknow campus, gomti nagar extension, lucknow up, india. Several biodegradable polymers have been studied as vaccine delivery vehicles 1. Generally, two main strategies are employed for their preparation.
The polyanhydride nanoparticles were then collected using vacuum filtration. Synthesis of copper oxide nanoparticles using simple chemical route jyoti mayekar, vijay dhar, s. Suspensions of particles in nanopure water were used. Polyanhydride nanoparticle delivery platform dramatically enhances killing of filarial worms. Study on nano polyaniline silver composite particles. Safety and biocompatibility of carbohydratefunctionalized. Jul 28, 2011 nano particles are molded in nano scale molds fabricated from nonwetting, low surface energy polymeric materials. Sem was used to assess size and morphology of the nanoparticle libraries. Polymer nanoparticles have attracted the interest of many research groups and have been utilized in an increasing number of fields during the last decades. In vivo, polyanhydrides degrade into nontoxic diacid monomers that can be metabolized and eliminated from the body. Cph nanoparticles better preserved the functionality and immunogenicity of pa during nanoparticle fabrication, storage, and release. Nanoparticle filled polymeric systems for gas barrier and. Polyanhydrides are one of the most considered and investigated biodegradable surfaceeroding polymers. Redoxresponsive polyanhydride micelles for cancer therapy.
Polyanhydride nanoparticle delivery platform dramatically. In vitro cytotoxicity studies with both immortal and primary cells demonstrated that the proposed polyanhydride blend is cytocompatible. The degradation rate of these particles was directly proportional to the ph of the surrounding medium, with particles degrading most rapidly at ph 8. Structural and antigenic stability of h5n1 hemagglutinin. Recently, polyanhydridebased nanocarriers has been approved by the fda for its application on cancer chemotherapy. Characterization of polyanhydride particles encapsulating ova. Using proper protective equipment and following decontaminationcleaning procedures, as described in nfpa 1851 should minimize the risk. Genetic factors driving attachment of bacteria to environmental particles, chunyu liao. Improving vaccine and immunotherapy design using biomaterials. We hypothesize that the mechanism behind this dramatically enhanced killing of the macrofilaria is the ability of the polyanhydride nanoparticles to behave as a trojan horse and penetrate the cuticle, bypassing excretory pump. All these systems prepared from bioerodible or biodegradable polymers polyanhydride, oester. Nadeemc adepartment of physics, quaidiazam university islamabad, pakistan.
Hemagglutininbased polyanhydride nanovaccines against h5n1. Synthesis of a polyanhydride nanoparticles based on polysa or 20. Pas are sebacic acids sas and their modified forms, polyanhydrideesters. Polyanhydride nanoparticle interactions with host serum proteins and their effects on bone marrow derived macrophage activation julia e.
Linear polyanhydrides with numberaverage molecular weights of several thousand g mol. Nano vaccines based on polyanhydride nanoparticles have been successfully used for the encapsulation and release of vaccine antigens. Synthesis of fe o nanoparticles by new solgel method and. Hydrophilic polymeric nanoparticles as drug carriers 275 particles get into circulation, but they are scavenged by the macrophages in blood, spleen and bone marrow. The use of transferringold nanoparticles as ligands, liposomal doxorubicin dox for interaction with folate receptors, and the application of magnetite encapsulated micro and nanoscale polymer particles have also been widely experimented 2022. Nano and microparticles as controlled drug delivery devices. Polyanhydrides are an important class of biodegradable polymers characterized by anhydride groups in the repeat unit of the polymer backbone. Transmission electron microscopy tem and atomic force microscopy afm revealed the seaisland morphology. These nanocapsules contain short strand dna, which when applied to water are absorbed into fish cells. The production of degradable polyanhydride based nanoparticles using thiolene click polymerizations is described. Polyanhydride particles were characterized in terms of size, shape, and surface charge. The design and fabrication of novel polyanhydride blend. Sem images representative of the chemistries studied are shown in fig.
Plga, plg, or poly lacticcoglycolic acid is a copolymer which is used in a host of food and drug administration fda approved therapeutic devices, owing to its biodegradability and biocompatibility. The drugloaded particles were characterized for size and. Shaw, polymer program, institute of materials science and department of chemical engineering, university of. Polyanhydrides erode via a surface erosion mechanism that limits the exposure of encapsulation payloads to water, and therefore, enhancing their stability 7. The nano particles can include pharmaceutical compositions, taggants, contrast agents, biologic drugs, drug compositions, organic materials, and the like. Neuronal protection against oxidative insult by polyanhydride. Initial studies included in vitro bioassays and in vivo material evaluation using a novel. Amphiphilic polyanhydride nanoparticles stabilize bacillus. Design of polyanhydridebased targeted nanovaccines against hiv. Jan 07, 2010 in some embodiments, the present invention provides methods for making isolated three dimensional geometric micro andor nano particles for inclusion in cosmetic applications or of cosmetic ingredients. Article pdf available in international journal of nanoparticles 54. In structure, the polymer has anhydride bonds that connect repeat units of the polymer backbone chain.
The effect of polyanhydride chemistry in particlebased. Their main application is in the medical device and pharmaceutical industry. Design of polyanhydridebased targeted nanovaccines against hiv, julia eulalia vela ramirez. Nano particles, asbestos and synthetic mineral fibers are all emerging as highly toxic products of combustion with fatal consequences. Hydrophilic polymeric nanoparticles as drug carriers.
Hemagglutininbased polyanhydride nanovaccines against. Polyanhydrides were first investigated in detail by hill and carothers 168 and were considered in the 1950s for possible applications as textile fibers 169. Nano silver polyaniline pmma composites were prepared by direct dispersion method. Polyanhydrides are a class of safe and biodegradable polymers that have. In this work, we investigated the delivery of biodegradable polyanhydride nanoparticles containing the mitochondriatargeted antioxidant apocynin to neuronal cells and the ability of the nano. Natural polymers the most commonly used natural polymers in. This study describes a highthroughput method using an automated robot for synthesizing polyanhydride nanoparticles encapsulating proteins. Us8927024b1 antimicrobial polyanhydride nanoparticles. Polyaniline was synthesized by the chemical synthesis. The use of amphiphilic polyanhydride nanoparticles as delivery vehicles presents a viable solution for longterm vaccine storage. Pdf the aim of this study was to develop and characterize the bioadhesive properties of polyanhydride nanoparticles coated with two types. Synthesis and characterization of crosslinked sulfonated.
In this work, the in vivo safety profile of carbohydratefunctionalized polyanhydride nanoparticles was analyzed following parenteral and intranasal administration in mice. Organically modified precursors are not susceptible to hydrolysis because an organic group is linked directly to a silicon atom, which does not need oxygen bridge. Polyanhydride nanovaccine platform against bacterial pathogens, shannon lee haughney. Sprayfreezing into scf produces micron or submicron protein particles. The anhydrides are highly susceptible to hydrolysis and degrade into nontoxic diacid monomers that can be metabolized and eliminated from the body, that is, many polyanhydrides are considered. Polyanhydride nanoparticles composed of sebacic anhydride and 1,6. Effects of alkaline extraction on micronano particles of. Polyanhydrides are useful bioabsorbable materials for controlled drug delivery. Silicabased mesoporous nanoparticles for controlled drug. These results demonstrate that polyanhydrides degrade more rapidly at high phs and are in accordance with a basecatalyzed hydrolysis mechanism. Pdf bioadhesive properties of polyanhydride nanoparticles. Nanoparticle vaccines against viral infections springerlink. Efficacy of mucosal polyanhydride nanovaccine against respiratory.
Plga is synthesized by means of ringopening copolymerization of two different monomers, the cyclic dimers 1,4dioxane2,5diones of. An increase in the gas barrier property was observed by adding silicate nanotubes or clay nanoparticles into polymeric systems. Synthesis of copper oxide nanoparticles using simple chemical. Drug reservoir is either encapsulated in microcapsules or homogeneously dispersed in microspheres or nano particles for injection. In this work, we investigated the delivery of biodegradable polyanhydride nanoparticles containing the mitochondriatargeted antioxidant apocynin to neuronal cells and the ability of the nanoformulation to protect cells against oxidative stress. Synthesis and characterization of crosslinked sulfonated polystyrene nanoparticles smita b. Respiratory protection for workers handling engineered. And the optimal concentrations of koh, naoh, lioh were 16. In this work, we investigated the delivery of biodegradable polyanhydride nanoparticles containing the mitochondriatargeted antioxidant apocynin to neuronal cells and the ability of the nano formulation to protect cells against oxidative stress. Their low hydrolytic stability, the major limitation for their industrial applications, was later recognized as a potential advantage by langer et al.
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